cell lines human pericytes  (PromoCell)

Journal: Cells

Article Title: Functional Expression of Multidrug-Resistance (MDR) Transporters in Developing Human Fetal Brain Endothelial Cells

doi: 10.3390/cells11142259

Figure Lengend Snippet: Tube formation in early and mid-gestation primary human fetal brain endothelial cells (hfBECs) . ( A ) early pregnancy hfBECs, ( B ) mid-pregnancy hfBECs, ( C ) adult endothelial cell line (hCMEC/D3) as positive control, and ( D ) human brain vessel pericyte cell line (HBVP). Tube formation was assessed after 24 h. n = 6/gestational age (representative images presented).

Article Snippet: Briefly, hCMEC/D3 cells were cultured at 20% O 2 (5% CO 2 , 37 °C) with EndoGRO™-MV Complete Culture Media Kit ® (SCME004, Millipore), human basic fibroblast growth factor (1 ng/mL; F0291, Sigma) and 1% penicillin-streptomycin (10,000 units-10,000 μg/mL, 15140-122, Life Technologies) for 24 h. Human Brain Vascular Pericytes cell line (HBVP) cells (4 passages; 1200, Sciencell Research Laboratories, Carlsbad, Canada) were cultured as previously described [ ].

Techniques: Positive Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Notch3 expression in capillary pericytes predicts worse graft outcome in human renal grafts with antibody‐mediated rejection

doi: 10.1111/jcmm.17325

Figure Lengend Snippet: Expression of Notch3 in normal grafts (A), and in grafts with antibody‐mediated rejection (ABMR) (B–D). (A) In normal kidneys, expression of Notch3 was mainly found to vascular smooth muscle cells (white arrows). No staining was found in peritubular capillaries. (B, C) During ABMR, a strong expression of Notch3 was observed in pericytes of peritubular capillaries (black arrows) closely attached to the endothelial cells (see inserts in the upper left and lower right corners for B and C, respectively). (D) Double staining with the vascular endothelial marker CD34 (red) and Notch3 (green) indicated that Notch3 was closely attached to and around the capillary endothelial cells in the biopsies with ABMR

Article Snippet: The human brain vascular pericytes (HBVPs) cell line (ScienCell) was cultured in pericyte medium (ScienCell) supplied with 100 U/ml penicillin/streptomycin (Gibco) on poly‐l‐lysine coated flasks according to the manufacturer's instructions.

Techniques: Expressing, Staining, Double Staining, Marker

Journal: Journal of Cellular and Molecular Medicine

Article Title: Notch3 expression in capillary pericytes predicts worse graft outcome in human renal grafts with antibody‐mediated rejection

doi: 10.1111/jcmm.17325

Figure Lengend Snippet: Notch3 expression evaluated by immnunohistochemistry in biopsies from patients with or without ABMR. To note that significantly higher levels of Notch3 pericyte expression was found in renal grafts with antibody‐mediated rejection (ABMR) compared to those without or in grafts with either no lesion or lesions due to other causes ( n = 13 for ABMR, n = 36 for other, p < 0.0001)

Article Snippet: The human brain vascular pericytes (HBVPs) cell line (ScienCell) was cultured in pericyte medium (ScienCell) supplied with 100 U/ml penicillin/streptomycin (Gibco) on poly‐l‐lysine coated flasks according to the manufacturer's instructions.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Notch3 expression in capillary pericytes predicts worse graft outcome in human renal grafts with antibody‐mediated rejection

doi: 10.1111/jcmm.17325

Figure Lengend Snippet: Correlations of Notch3 expression in pericytes and of pEndMT markers in endothelial cells of peritubular capillaries with the clinic and morphological lesions

Article Snippet: The human brain vascular pericytes (HBVPs) cell line (ScienCell) was cultured in pericyte medium (ScienCell) supplied with 100 U/ml penicillin/streptomycin (Gibco) on poly‐l‐lysine coated flasks according to the manufacturer's instructions.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Notch3 expression in capillary pericytes predicts worse graft outcome in human renal grafts with antibody‐mediated rejection

doi: 10.1111/jcmm.17325

Figure Lengend Snippet: Human pericytes cultured under control conditions or after administration of IL‐6 or INFγ. To note that both cytokines strongly induced Notch3 expression. This increase was accompanied by concomitant increase of several inflammatory and fibrotic markers, including MCP‐1, Rage9, ICAM‐1 and VCAM‐1 and the pro‐migratory integrin β3. (* p < 0.05; ** p < 0.01, n = 4)

Article Snippet: The human brain vascular pericytes (HBVPs) cell line (ScienCell) was cultured in pericyte medium (ScienCell) supplied with 100 U/ml penicillin/streptomycin (Gibco) on poly‐l‐lysine coated flasks according to the manufacturer's instructions.

Techniques: Cell Culture, Control, Expressing

Journal: Micromachines

Article Title: Recent Progress in Microfluidic Models of the Blood-Brain Barrier

doi: 10.3390/mi10060375

Figure Lengend Snippet: Cellular constituents of the blood-brain barrier (BBB). The BBB is formed by brain microvascular endothelial cells (BMECs), which are connected by tight junctions. The endothelium, together with the basal lamina, pericytes, and astrocytic end-feet forms the neurovascular unit. Some substances diffuse freely into and out of the brain parenchyma, others such as nutrients need specific transporters, while molecules such as insulin, leptin and transferrin are transported by receptor- mediated transcytosis. (Reprinted from Reference in accordance with the Creative Commons Attribution).

Article Snippet: Ref. [ ] , High-throught, the model harbors 96 or 40 chips in a 384-well plate. In each chip, a perfused vessel of BMECs was grown against an extracellular matrix gel, astrocytes and pericytes were added on the other side of the gel to complete the BBB model. , Human TY10 cell line (isolated from normal brain tissue from a patient with meningioma) , Human hBPCT cell line pericytes from brain tissue of a patient t. Human hAst cell line astrocytes from human primary astrocytes distributed by Lonza. , No artificial membranes, using extracellular matrix gel , Collagen-I , No electrode , claudin-5, VE-cadherin, PECAM-1 (immunofluorescence staining) , Not measured , FITC-dextran (20 kDa) , Developed a high-throughput plate-based model, and used to assess passage of large biopharmaceuticals across the BBB..

Techniques:

Journal: Micromachines

Article Title: Recent Progress in Microfluidic Models of the Blood-Brain Barrier

doi: 10.3390/mi10060375

Figure Lengend Snippet: The main characters of some microfluidic blood-brain barrier (BBB) models.

Article Snippet: Ref. [ ] , High-throught, the model harbors 96 or 40 chips in a 384-well plate. In each chip, a perfused vessel of BMECs was grown against an extracellular matrix gel, astrocytes and pericytes were added on the other side of the gel to complete the BBB model. , Human TY10 cell line (isolated from normal brain tissue from a patient with meningioma) , Human hBPCT cell line pericytes from brain tissue of a patient t. Human hAst cell line astrocytes from human primary astrocytes distributed by Lonza. , No artificial membranes, using extracellular matrix gel , Collagen-I , No electrode , claudin-5, VE-cadherin, PECAM-1 (immunofluorescence staining) , Not measured , FITC-dextran (20 kDa) , Developed a high-throughput plate-based model, and used to assess passage of large biopharmaceuticals across the BBB..

Techniques: Permeability, Immunofluorescence, Staining, Derivative Assay, Diffusion-based Assay, Marker, In Vivo, Western Blot, Microscopy, Isolation, Impedance Spectroscopy